The transcriptome sequence analysis of <em>Artemisia frigida</em> — ASN Events
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  3. The transcriptome sequence analysis of Artemisia frigida

The transcriptome sequence analysis of Artemisia frigida (#256)

Yue Liu 1 2 3 4 , Xiaoxiao Feng 1 , Yi Wang 3 4 , Naxin Huo 3 4 , Xu Ma 1 , Chuanchuan Chen 1
  1. Minzu University of China, Beijing, China
  2. National Resource Center for Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, China
  3. USDA-ARS, Western Regional Research Center, Albany, CA, USA
  4. Department of Plant Science, University of California, Davis, CA, USA

Artemisia frigida, also named as Xiaobaihao or Hanhao, is a Mongolian traditional medicinal plant. It belongs to the genus of Artemisia in Asteraceae family. This plant has the power of stanch, detumescence and is well applied in the cure of illness, such as bleeding, arthroncus, colds, coughs, mountain fever, and so on. Besides its medical efficacy, it is also valued as a very important feeding resource, and a cultural symbol in the daily life of the Mongolian and indigenous peoples of America.

Three tissues from sample N01 of Artemisia frigida was carried out using Illumina 100 bp paired-end sequencing technology on the Illumina HiSeq 2000 system. A total of 15,938,471,800 bp from 159,320,702 reads of three Artemisia frigida samples were obtained. After removing the low quality sequence, we got 12,317,103,117 bp of data which accounted for 77.28% of the raw data. These clean data were assembled into 21,221, 32,339 and 9,028 unigenes in the three samples of Artemisia frigida, leaf, stem and root, respectively. The N 50 length of the unigenes in each sample ranged from 598 bp to 808 bp and the mean length of the unigenes ranged from 575 bp to 713 bp. Then we did the transcriptome analysis of Artemisia frigida, including gene annotation, functional classification and differential expression, etc. According to the analysis, we found that the gene expression level of flavonoids biosynthesis enzymes, such as phenylalnine ammonialyase, cinnamic acid-4-hydroxylase, chalcone synthase, chalcone isomerase and flavone synthase II and so on, was as follows: the gene expression level in the stem was higher than that in the leaf, while that in the leaf was higher than that in the root. Therefore, we speculated that the most suitable medicinal parts of Artemisia frigida were leaf and stem.

In this study, the transcriptome of Artemisia frigida was analyzed, which enriched the genetic information of Artemisia frigida, and helped study flavonoids secondary metabolic pathways in this plant. Also, it will lay a foundation for improving the active ingredients of ethnic medicines by using genetic engineering in the future.