Comparative analysis of gene expression profiles induced by IL-4 and IL-6 in human peripheral blood mononuclear cells — ASN Events
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  3. Comparative analysis of gene expression profiles induced by IL-4 and IL-6 in human peripheral blood mononuclear cells

Comparative analysis of gene expression profiles induced by IL-4 and IL-6 in human peripheral blood mononuclear cells (#272)

Gaopeng Li 1 , Hongwu Du 2 , Guangyu Chen 3 , Charles Fathman 3 , Yan Zhang 1
  1. Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
  2. School of Chemistry & Biotechnology Engineering, University of Science & Technology Beijing, Beijing, China
  3. Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA, USA

Background: IL-4 and IL-6 play important roles in a variety of biological processes such as inflammation and immune response. Although the crosslinks between these two molecules have been reported previously, little work has been done at the systematic level to investigate their joint effects on different cells.

Results: We carried out microarray analysis of human peripheral blood mononuclear cells with IL-4 and IL-6 stimuli at different time points and under different culture conditions (hydrogen peroxide or phytohaemagglutinin). A large number of differentially expressed genes were detected within each condition, and were enriched in a variety of GO terms and pathways. Investigation of some representative pathways revealed that different conditions may influence the same pathway; however, the effects were quite different. PPI network analysis was also performed for identification of key regulators and condition-specific genes.

Conclusions: Our data provide new insights into the complex roles of IL-4 and IL-6 as well as their cellular effects under different conditions.